Studies in the Respiratory and Carbohydrate Metabolism of Plant Tissues1: XVI. FURTHER STUDIES OF THE INFLUENCE OF IODOACETATE AS A STIMULANT OF RESPIRATION IN STRAWBERRY LEAVES

Low concentrations of iodoacetate produced large increases inCO₂ output of strawberry leaves which were not completely accountedfor by the observed losses of sugars and starch. No significantchange was observed in RQ in iodoacetate but the values, eitherin water or in iodoacetate, were generally below unity. Thus,in addition to other carbohydrates not estimated in our work,there may also be respiration of protein or organic acids. The increased rate of CO₂, output in iodoacetate was associatedwith rises in pyruvate and oxaloacetate but citrate and -ketoglutarateremained unchanged. The former changes suggested a faster trafficinto the tricarboxylic acid cycle; the content of oxaloacetateappeared to be a close index of the rate of this traffic. Atentative interpretation is given of the anomalous behaviourof citrate and -ketoglutarate in iodoacetate.