An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development |
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Authors: | Karen E Reid Niclas Olsson James Schlosser Fred Peng and Steven T Lund |
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Institution: | (1) Faculty of Land and Food Systems, University of British Columbia, Vancouver, Canada |
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Abstract: | Background Accuracy in quantitative real-time RT-PCR is dependent on high quality RNA, consistent cDNA synthesis, and validated stable
reference genes for data normalization. Reference genes used for normalization impact the results generated from expression
studies and, hence, should be evaluated prior to use across samples and treatments. Few statistically validated reference
genes have been reported in grapevine. Moreover, success in isolating high quality RNA from grapevine tissues is typically
limiting due to low pH, and high polyphenolic and polysaccharide contents. |
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Keywords: | |
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