H-NS controls metabolism and stress tolerance in Escherichia coli O157:H7 that influence mouse passage |
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| Authors: | Irfan Erol Kwang-Cheol Jeong David J Baumler Boris Vykhodets Sang Ho Choi Charles W Kaspar |
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| Institution: | (1) Department of Food Hygiene and Technology, University of Ankara, Ankara, Turkey;(2) Department of Food Microbiology and Toxicology, University of Wisconsin, 53706-1187 Madison, Wisconsin, USA;(3) Cellular and Molecular Biology, University of Wisconsin, 53706-1187 Madison, Wisconsin, USA;(4) Department of Food Science and Technology, Seoul National University, 151-742 Seoul, South Korea |
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| Abstract: | Background H-NS is a DNA-binding protein with central roles in gene regulation and nucleoid structuring in Escherichia coli. There are over 60 genes that are influenced by H-NS many of which are involved in metabolism. To determine the significance
of H-NS-regulated genes in metabolism and stress tolerance, an hns mutant of E. coli O157:H7 was generated (hns::nptI, FRIK47001P) and its growth, metabolism, and gastrointestinal passage compared to the parent strain (43895) and strain FRIK47001P
harboring pSC0061 which contains a functional hns and 90-bp upstream of the open-reading frame. |
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