Temporal and spatial expression patterns of two G-protein coupled receptors inDrosophila melanogaster |
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Authors: | Frances Hannan Linda M Hall |
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Institution: | (1) Present address: Department of Zoology, The Babraham Institute Laboratory of Molecular Signalling, CB2 3EJ Cambridge, UK;(2) Department of Biochemical Pharmacology, State University of New York at Buffalo, 329 Hochstetter Hall, 14260-1200 Buffalo, NY, USA |
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Abstract: | Temporal and spatial expression patterns of a muscarinic acetylcholine receptor (Acr60C) and an octopamine/tyramine receptor (Octyr) were determined inDrosophila melanogaster using quantitative Northern analysis andin situ hybridization to tissue sections. Expression of mRNA encoding both of these G-protein coupled receptors peaks initially in
18 to 21 hour embryos following the formation of the mature larval nervous system. Levels of mRNA then decline during larval
stages, rising to a second peak in 3 to 4-day-old pupae after a period of major nervous system reorganization. The muscarinic
acetylcholine receptor mRNA is expressed throughout the cortical regions of the central nervous system in adults and embryos.
Particularly high levels of expression of Acr60C are observed in cell bodies adjacent to the antennal lobes, suggesting a major role for this muscarinic receptor in the
processing of olfactory information. In contrast, the octopamine/tyramine receptor mRNA is distributed diffusely throughout
the adult brain, with patches of signal concentrated in the cortex of the dorsal protocerebrum near the mushroom bodies. These
patches may represent individual cells expressingOctyr receptors. |
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Keywords: | muscarinic acetylcholine receptor octopamine/tyramine receptor developmental profile in situ hybridization antennal lobes mRNA transport |
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