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Sequential resonance assignments as a basis for determination of spatial protein structures by high resolution proton nuclear magnetic resonance
Authors:Kurt Wüthrich  Gerhard Wider  Gerhard Wagner  Werner Braun
Affiliation:Institut für Molekularbiologie und Biophysik Eidgenössische Technische Hochschule ETH-Hönggerberg, CH-8093 Zürich, Switzerland
Abstract:A general scheme is proposed for the determination of spatial protein structures by proton nuclear magnetic resonance. The scheme relies on experimental observation by two-dimensional nuclear magnetic resonance techniques of complete throughbond and through-space proton-proton connectivity maps. These are used to obtain sequential resonance assignments for the individual residues in the amino acid sequence and to characterize the spatial polypeptide structure by a tight network of semi-quantitative, intramolecular distance constraints.
Keywords:n.m.r.  nuclear magnetic resonance  COSY  2dimensionalcorrelated spectroscopy  SECSY  2-dimensional spin echo correlated spectroscopy  NOE  nuclear Overhauser enhancement  NOESY  2-dimensional NOE spectroscopy
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