HineI is an isoschizomer of HinfIII restriction endonuclease

HineI is a restriction enzyme isolated from Haemophilus influenzae strain Re. Like other type III restriction endonucleases it requires ATP for cleavage and S-adenosyl-methionine for methylation of DNA. This enzyme recognises the same sequence as HinfIII (Piekarowicz et al., 1981) and cleaves and methylates DNA in a manner similar to all type III restriction enzymes.