ATP production by sorbitol-treated cells of a methanol yeast, Candida boidinii (Kloeckera sp.) No. 2201 |
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| Authors: | Yoshiki Tani Tetsu Yonehara Yukio Mitani Hideaki Yamada |
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| Affiliation: | 1. Research Center for Cell and Tissue Culture, Kyoto University, Kyoto 606, Japan;2. Department of Agricultural Chemistry, Kyoto University, Kyoto 606, Japan |
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| Abstract: | The phosphorylation system of AMP by sorbitol-treated cells of a methanolutilizing yeast, Candida boidinii (Kloeckera sp.) No. 2201, was investigated for the production of ATP. Firstly, reaction conditions for the ATP production were optimized. Under the optimal conditions, 20–30 g 1?1 of ATP were produced in the conversion rate of 60–70% from AMP. Activities of reactions involved in the ATP-producing system were compared with cells from different cultures to prepare the cells having the higher activity and to know the essential reaction limiting the rate of the system. The energy efficiency of this system was also discussed. |
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| Keywords: | methanol yeast ATP production by yeast sorbitol-treated yeast cells |
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