Unordered arrangement of chromosomes in the nuclei of chicken spermatozoa |
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| Authors: | Irina V Solovei Boris I Joffe Tetsua Hori Philippa Thomson Shigeki Mizuno Herbert C Macgregor |
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| Institution: | (1) Institute of Anthropology and Human Genetics, University of Munich, Munich, Germany, DE;(2) Zoological Institute RAN, St Petersburg, Russia, RU;(3) Laboratory of Molecular Biology, Department of Applied Biological Chemistry, Tohoku University, Japan, JP;(4) Department of Biology, University of Leicester, Leicester, LE1 7RH, England, GB |
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| Abstract: | The arrangement of chromosomes in the elongated sperm nuclei of chicken was studied using fluorescence in situ hybridization
with probes specific for telomeres of all chromosomes, a microchromosome, the long arm of chromosome 6, the large heterochromatic
block on the Z-chromosome, and the same heterochromatic block plus subtelomeric sites on macrochromosomes 1–4. The positions
of all probes vary from one sperm to another. No order in chromosome arrangement is apparent. It is suggested that large chromosome
size and small chromosome number correlate with constant positions of chromosomes and vice versa. Based on the known quantity
of repetitive units of the repeat on the Z-chromosome, the degree of compaction of chromatin in the chicken sperm nucleus
is estimated as ca 0.7 Mb/μm. As judged from the length of the heterochromatic region of the Z-chromosome at the lampbrush
stage, the total length of the Z-chromosome in mature sperm is 2.5–4 times that of the sperm nucleus.
Received: 15 December 1997; in revised form: 24 March 1998 / Accepted: 14 April 1998 |
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