The SecA Subunit of Escherichia coli Preprotein Translocase Is Exposed to the Periplasm |
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Authors: | Jerry Eichler and William Wickner |
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Affiliation: | Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755-3844 |
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Abstract: | SecA undergoes conformational changes during translocation, inserting domains into and across the membrane or enhancing the protease resistance of these domains. We now show that some SecA bound at SecYEG is accessible from the periplasm to a membrane-impermeant probe in cells with a permeabilized outer membrane but an intact plasma membrane. |
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