Germplasm resources research of Toona sinensis with RAPD and isoenzyme analysis |
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Authors: | Changlu Wang Junwu Cao Shaoran Tian Yurong Wang Zhiqiang Chen Mianhua Chen Guoli Gong |
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Institution: | (1) College of Food Engineering & Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, People’s Republic of China;(2) College of Bioengineering, Tianjin University of Science & Technology, Tianjin, 300457, People’s Republic of China |
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Abstract: | Collected from Chinese mainland, 25 samples of Toona sinensis seed were planted in a standard garden. Three years later, samples were analyzed with random amplified polymorphic DNA (RAPD)
and isoenzymes variants of cytochrome oxidase and peroxidases. Results of RAPD and isoenzymes variants were analyzed with
software POPGENE32 and SPSS10.0 respectively. Phylogenetic trees were calculated from the clustered result of RAPD and isoenzymes
variants. Such additional factors as leaves color, latitude and altitude of original seeds were analyzed at the same time.
Our result suggested that a general similarity is concurred with the three analyses as RAPD, isoenzymes variants analysis
of peroxidases and cytochrome oxidase. Two clusters can be separated from the sample collected as southern and northern ones.
In the evolutionary history, a migrating route in the direction from West-South to East-North and with sequential differentiation
in the direction South-North can be decided if only it is true with the argument that Toona sinensis came from tropic Asia, the border of east-north India and Burma. |
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Keywords: | Toona sinensis RAPD germplasm resources isoenzyme analysis |
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