Growth of an adherent continuous cell line entrapped in a peg/alginate matrix |
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| Authors: | Gary J. Gates Janice A. Phillips |
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| Affiliation: | (1) Department of Chemical Engineering, Lehigh University, Iacocca Hall 111 Research Drive, 18015 Bethlehem, PA;(2) BioProcessing Institute, Lehigh University, Iacocca Hall 111 Research Drive, 18015 Bethlehem, PA;(3) Present address: SmithKline Beecham Pharmaceuticals, 709 Swedeland Road, 19406 King of Prussia, PA |
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| Abstract: | Summary CHO-K1 cells, an anchorage-dependent line, were entrapped in beads prepared from a Na alginate/polyethylene glycol mixture and grown, through successive passages, to an average maximum density of 4.5×107 viable cells/g of bead. Cell growth and viability was unaffected by repeated alginate re-solubilization and reformation of the gel beads through five passages. |
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