Characterization of PAF receptors on human neutrophils using the specific antagonist, WEB 2086. Correlation between receptor binding and function |
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| Authors: | G Dent D Ukena P Chanez G Sybrecht P Barnes |
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| Institution: | Department of Thoracic Medicine, National Heart & Lung Institute, London, England. |
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| Abstract: | The antagonism of PAF effects by WEB 2086 and the receptor binding of 3H]WEB 2086 were investigated in isolated human neutrophils. WEB 2086 inhibited PAF-induced beta-glucuronidase release and 3H]WEB 2086 bound specifically to high-affinity sites on the cells. Close concordance between affinity constants for WEB 2086 from functional and radioligand-binding studies suggests that WEB 2086 interacts with the neutrophil PAF receptors and that 3H]WEB 2086 may be a useful ligand in investigation of these receptors. |
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