Composition of an isoelectric focusing gel yielding a broad pH gradient. |
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Authors: | T B Breithaupt I E Nystrom D H Hodges J A Babitch |
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Affiliation: | Department of Chemistry and Chemistry of Behavior Program Texas Christian University Fort Worth, Texas 76129 USA |
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Abstract: | An isoelectric focusing gel system is described which produces a pH gradient spanning the range 4–9. When chick brain mitochondrial polypeptides were focused on such a gel, extra polypeptide spots were observed in the basic region which were not seen in a gel prepared by conventional methods. |
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