Esr and Spin-Trapping Study of Free Radicals in γ-Irradiated Solid Lysozyme |
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Authors: | Osamu Inanami Mikinori Kuwabara Fumiaki Sato |
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Affiliation: | a Department of Radiation Biology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan |
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Abstract: | Free radicals produced by γ-irradiation of solid lysozyme were investigated by a technique combining ESR, spin-trapping and enzymatic digestion. MNP and DMPO were used as spin-trapping reagents. The solid lysozyme was first γ-irradiated and then dissolved in an aqueous solution containing the spin-trapping reagent to stabilize free radicals. The spin adducts of lysozyme were digested to oligopeptides to get ESR spectra having a well-resolved hyperfine structure. The ESR spectra obtained showed that carbon-centered radicals, CH-, at the side chains of amino acids, and thiyl radicals, -CH2--S-, at disulfide bridges were produced in γ-irradiated solid lysozyme. |
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Keywords: | ESR spin-trapping lysozyme free radicals |
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