Human and animal mesenchymal progenitor cells from bone marrow: Identification of serum for optimal selection and proliferation |
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Authors: | Donald P Lennon Stephen E Haynesworth Scott P Bruder Neelam Jaiswal Arnold I Caplan |
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Institution: | (1) Present address: Skeletal Research Center, Department of Biology, Case Western Reserve University, 2080 Adelbert Road, 44106-7080 Cleveland, Ohio;(2) Present address: Department of Orthopaedics, Case Western Reserve University, 2080 Adelbert Road, 44106-7080 Cleveland, Ohio;(3) Present address: Osiris Terapeutics, Inc., Baltimore, Maryland |
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Abstract: | Summary An undifferentiated subset of cells within the stromal cell population of bone marrow in postnatal mammals retains the capacity
to differentiate along osteogenic, adipogenic, fibroblastic, and chondrogenic lines. These cells, which are referred to as
mesenchymal stem cells (MSCs), can be maintainedin vitro and expanded in number through a process of subculturing. MSCs are maintained in culture in medium supplemented with 10%
fetal bovine serum (FBS). It is believed that certain, as yet unidentified, serum components play critical roles in the attachment
and proliferation of MSCs. Commercially available FBS is poorly characterized and may vary in composition and quality from
lot to lot. This study describes a method for the selection of lots of FBS that best support maintenance of the undifferentiated
state, mitotic expansion of MSCsin vitro, and retention of multilineage developmental potential in response to appropriate cues. |
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Keywords: | fetal bovine serum proliferation mesenchymal stem cell marrow osteochondral potential |
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