|
|||||
|
|
DNA-protein cross-linking in L1210 cells sensitive and resistant to cis-diamminedichloroplatinum (II) |
|
| Authors: | Ryszard Olinski Robert C. Briggs |
| Affiliation: | (1) Department of Clinical Biochemistry, Medical School, M. Sklodowskiej-Curie 9, 85-094 Bydgoszcz, Poland;(2) Department of Pathology and Biochemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, 37232 Nashville, Tennessee, USA |
| Abstract: | The role of restricted cellular accumulation of cis-diamminedichloroplatinum(II) (cis-DDP) and altered repair of DNA-Pt-protein cross-links in the mechanism of L1210 murine leukemia cell resistance was examined. An immunochemical method was used to analyze the formation and removal of DNA-Pt-protein complexes in L1210 cells sensitive and resistant to cis-DDP. The accumulation of Pt into the cells and the binding of Pt to the DNA was measured by atomic absorption spectroscopy. The results demonstrated that both decreased accumulation of the drug and the rate of DNA-Pt protein cross-link removal may be important factors in L1210 cell resistance to cis-DDP.Abbreviations AAS atomic absorption spectroscopy - cis-DDP cis-diamminedichloroplatinum(II) |
| Keywords: | cell resistance cis-DDP DNA-protein cross links |
| 本文献已被 SpringerLink 等数据库收录！ | |