大孔吸附树脂对紫锥菊提取物中菊苣酸分离纯化的研究 |
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引用本文: | 曾栋,陈波,罗旭彪,张斐,姚守拙. 大孔吸附树脂对紫锥菊提取物中菊苣酸分离纯化的研究[J]. 天然产物研究与开发, 2004, 16(2): 160-162 |
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作者姓名: | 曾栋 陈波 罗旭彪 张斐 姚守拙 |
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作者单位: | 湖南师范大学化学化工学院,长沙,410081 |
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基金项目: | 国家“十五”攻关项目 ( 2 0 0 1BA746C),湖南省重点项目 ( 2 0 0 1BA80 4A2 1),湖南省自然科学基金( 0 2JJY2 0 2 3 ) |
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摘 要: | 采用大孔吸附树脂分离纯化紫锥菊提取物中免疫活性成分菊苣酸,7%(v/v)的甲醇-水溶液洗脱,HPLC法对产品中菊苣酸含量进行检测分析。该法能把紫锥菊提取物中菊苣酸含量(4%)提高9倍左右,回收率达到95%以上,且操作简单,可用于菊苣酸的分离纯化。
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关 键 词: | 大孔吸附树脂 紫锥菊 提取物 菊苣酸 分离 纯化 免疫活性成分 |
修稿时间: | 2003-06-25 |
STUDY ON ADSORPTION AND PURIFICATION OF CICHORIC ACID IN EXTRACTS OF ECHINACEA PURPUREA WITH MACROPOROUS ADSORPTION RESIN |
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Abstract: | Developed a method to separate and enrich the immunocompetence constituent cichoric acid from the extracts of Echinacea purpurea with macroporous adsorption resin,by eluting with 7% (v/v) CH 3OH-H 2O. Cichoric acid content in product quantified by HPLC was nine times higher than that (4%) of the extracts of Echinacea purpurea. The recovery of cichoric acid was more than 95%. The technology was simple and could be applied to separate and purify cichoric acid. |
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Keywords: | macroporous adsorption resin extracts of Echinacea purpurea cichoric acid |
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