Synthesis of Acid Protease from Mucor miehei: Integration of Production and Recovery |
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Authors: | Jose Escobar and Stanley Barnett |
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Institution: | a Department of Chemical Engineering, University of Rhode Island Kingston, RI 02881 USA |
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Abstract: | The production of acid protease from Mucor Miehei was repressed by amino acid additions (3·5-5·0 g/litre) from hydrolysed casein. The integration of the batch bioprocess with an ultrafiltration unit proved effective in depressing enzyme production. A hollow fibre ultrafilter with a 5000 MW cutoff was used to reduce the concentration of amino acids present in the medium after enzyme concentration began to decline. Volume concentrating ratio (VCR) values of 2 and 3 were used for the experiments. Increases as high as 25% in enzyme concentration were achieved. |
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