Lactate dehydrogenase isoenzymatic analysis of murine lymphocyte populations: a parameter of cellular differentiation. |
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| Authors: | J Plum S Ringoir M de Smedt E Dhont |
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| Institution: | 1. Department of Medical Microbiology, University Hospital, Ghent, Belgium;2. Department of Medicine, Division of Nephrology, University Hospital, Ghent, Belgium |
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| Abstract: | The lactate dehydrogenase isoenzyme pattern has been determined in different murine lymphocytic cell populations. In each cell population, the LDH activity was predominantly found in the LDH-4 and LDH-5 fractions. The percentage LDH-5 activity was significantly higher in B cells than in T cells. The same is true for lymphocytes from the spleen versus lymph node lymphocytes. The percentage LDH-5 activity is significantly higher in peripheral T lymphocytes than in thymocytes. Enrichment of the more mature thymocytes of the thymocyte cell pool by either cortisone treatment in vivo or gradient centrifugation on bovine serum albumin (BSA) results in a decrease of LDH-1 and LDH-2 fractions. In the cortisone-treated group, the shift in the LDH pattern is accompanied by a significant increase of LDH-5 and LDH-4 fractions, whereas in the BSA group only the LDH-4 fraction increases. |
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| Keywords: | LDH lactate dehydrogenase B cell antibody-forming cell precursor T cell thymus-derived cell RPMI 1640 Roswell Park Memorial Institute BSA bovine serum albumin Ig immunoglobulin |
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