Abdominal angiostrongyliasis in rodent experimental infection: evidence for systemic circulation of first stage larvae |
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| Authors: | Fontoura G D R Maurer R L Oliveira C M Graeff-Teixeira C |
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| Affiliation: | 1. Laboratorio de Zoonosis Parasitarias, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350, 7600 Mar del Plata, Argentina;2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina;1. Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN) and Global COE Program, Nagasaki University, 1-12-4 Sakamoto, 852-8523, Japan;2. Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, 852-8523, Japan;3. Section of Environmental Parasitology, Tokyo Medical and Dental University Graduate School of Medical and Dental Science, 113-8519, Japan;4. Department of Hospital Pharmacy, Nagasaki University Hospital, 1-7-1 Sakamoto, 852-8501, Japan;5. Department of Clinical Product Development, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Japan;6. Department of Parasitology and Entomology, Faculty of Bioscience, Nnamdi Azikiwe University, P.M.B. 5025, Awka, Nigeria;1. Curso de Pós graduação em Medicina Tropical, Instituto Oswaldo Cruz, Fiocruz, Brazil;2. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz, Fiocruz, RJ, Brazil;3. Departamento de Ciências Fisiológicas, Instituto de Biologia and Curso de Pós Graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, BR-465-km 7, CEP 23890-000 Seropédica, Rio de Janeiro, Brazil;4. Laboratório de Patologia, Instituto Oswaldo Cruz, Fiocruz, Av. Brasil 4365, CEP 21040-30 Rio de Janeiro, Brazil;5. Departamento de Controle de Qualidade Animal, Centro de Criação de Animais de Laboratório (CECAL), Fundação Oswaldo Cruz, Rio de Janeiro, Brazil;1. Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan;2. Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan;3. Department of Animal Risk Management, Faculty of Risk and Crisis Management, Chiba Institute of Science, 15-8 Shiomi, Choshi, Chiba 288-0025, Japan;4. Center for Laboratory Animal Science, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan;5. Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan;1. Medical Parasitology Department, Faculty of Medicine, Mansoura University, Egypt;2. Department of Clinical Pharmacology, Faculty of Medicine, Mansoura University, Egypt;3. Department of Pathology, Faculty of Medicine, Mansoura University, Egypt |
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| Abstract: | Eggs of Angiostrongylus costaricensis embrionate and hatch in the course of their migration in the intestinal wall, and first stage larvae (L1) are released in feces. Aiming to investigate the possible systemic circulation of L1, we inoculated mice and, four weeks later, examined their peritoneal cavities and several organs for the presence of L1. A total of 65 larvae were found in extra-intestinal organs (kidney, heart, spleen, liver, lungs). No larvae were found in blood or in the peritoneal cavity. 320 and 578 L1 were found in intestinal wall and intestinal contents, respectively. The experiment was repeated and it confirmed that the metastrongylid larvae found in several organs were larval stages of A. costaricensis. Ten Oligoryzomis sp. rodents, a natural host for A. costaricensis, were also infected and in three animals L1 could be recovered from several organs as well as from bronchoalveolar lavage (BAL) in one of them. These data indicate that systemic circulation and bronchial elimination of L1 may represent an alternative route for release of L1 of A. costaricensis into the environment for transmission to the intermediate host. |
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