Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood |
| |
Authors: | Udomsangpetch Rachanee Somsri Sangdao Panichakul Tasanee Chotivanich Kesinee Sirichaisinthop Jeeraphat Yang Zhaoqing Cui Liwang Sattabongkot Jetsumon |
| |
Affiliation: | Department of Pathobiology, Mahidol University, Bangkok, Thailand. scrud@mahidol.ac.th |
| |
Abstract: | Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |