Up-Regulation of Licochalcone A Biosynthesis and Secretion by Tween 80 in Hairy Root Cultures of Glycyrrhiza uralensis Fisch |
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Authors: | Hai-Chao Zhang Jing-Mei Liu Hai-Min Chen Chun-Chun Gao Hong-Yu Lu Hua Zhou Yi Li Shan-Lin Gao |
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Affiliation: | (1) Department of Breeding and Genetics, China Pharmaceutical University, 210038 Nanjing, Jiangsu Province, China;(2) National Centre for Molecular Crop Design, 100085 Beijing, China;(3) Food Science and Technology College, Guangdong Ocean University, 524025 Zhanjiang, Guangdong Province, China;(4) The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871, China; |
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Abstract: | We evaluated the effect of Tween 80 as elicitor on licochalcone A from hairy root cultures of Glycyrrhiza uralensis Fisch. After a 15-days treatment with 2% Tween 80, hairy roots still grew well and produced higher levels of licochalcone A and total flavonoids than the control (without treatment). Licochalcone A content and total flavonoid content were 3.103 and 127.095 mg per flask (9- and 11-fold higher), respectively, compared with controls. Secretion of licochalcone A and total flavonoids into the culture medium was remarkably high, up to 98 and 94% of the total production, respectively. The enhanced flavonoid production was associated with elevated mRNA levels and enzyme activities of phenylalanine ammonia-lyase (PAL), 4-coumarate:coenzyme A ligase (4CL), and cinnamate-4-hydroxylase (C4H). These results clearly demonstrated that Tween 80 treatment permeabilized the roots to enhance secretion, but also acted as an efficient elicitor of licochalcone A and total flavonoid production in hairy roots of G. uralensis Fisch. |
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