Community level physiological study of algicidal bacteria in the phycospheres of Skeletonema costatum and Scrippsiella trochoidea |
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| Affiliation: | 1. Institute of Hydrobiology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;2. Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;1. MARBEC, CNRS IRD IFREMER Univ Montpellier, Sète, France;2. LBE, INRA, Univ Montpellier, Narbonne, France;3. Present address: Institut de biosciences et biotechnologies, CEA Cadarache, St Paul Lez Durance, France;1. Rutgers, The State University of New Jersey, Institute of Marine and Coastal Sciences, 71 Dudley Road, New Brunswick, NJ 08901, USA;2. Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL 34236, USA;3. Virginia Institute of Marine Science, College of William & Mary, P.O. Box 1346, Gloucester Point, VA 23062, USA;1. School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China;2. Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, Xi’an 710055, China;1. Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, School of Environment and Energy, South China University of Technology, Guangzhou 510006,Guangdong, China;2. Department of Chemistry, Jinan University, Guangzhou 510632, Guangdong, China |
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| Abstract: | Bacteria in the phycosphere have a unique ecological relationship with host algae due to their utilization of algal extracellular products as nutrients. Some bacteria control the growth of algal cells and even lyse them. The diversity of bacteria and their community dynamics in the phycosphere of microalgae are still relatively little understood, especially of those associated with red tide-causing algae. In this study, scanning electron microscope (SEM) images of algal cell morphology revealed that the phycosphere bacteria of the red tide-causing algae, Skeletonema costatum and Scrippsiella trochoidea, could lyse them within 72 h. The community level physiology of the algicidal bacteria was studied using Biolog ECO microplates, a common method for the ecological study of microbial communities. The average well color development (AWCD) values of bacteria in the phycospheres of both species were low, indicating that the bacteria had low metabolic activity overall. The diversity indices were both lower than the bacterial diversity from natural environments. However, the bacteria associated with S. trochoidea demonstrated a higher AWCD value and diversity than those in the phycosphere of S. costatum. The utilization of carbon sources significantly changed at different lytic times, reflecting that the bacterial community structure changed during the algae-lysing process. These results revealed that the bacterial communities in phycospheres had a simple structure and low diversity. When the balance between algae and bacteria broke down, the total bacterial density increased while the algicidal bacteria accumulated and became the dominant species, changing the bacterial community structure in this micro-ecosystem. |
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| Keywords: | Algicidal bacteria Community level physiology Phycosphere Red tide-causing algae |
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