Ostreopsis cf. siamensis and Ostreopsis cf. ovata from the Atlantic Iberian Peninsula: Morphological and phylogenetic characterization |
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| Affiliation: | 1. Department of Plant Biology and Ecology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain;2. SGIKer Genomic Service, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain;1. IRTA, Carretera de Poble Nou, km 5.5, 43540 Sant Carles de la Ràpita, Spain;2. IFREMER, DYNECO-PELAGOS Centre de Brest, Pointe du Diable BP70, 29280 Plouzane, France;1. Dipartimento di Scienze della Vita e dell’Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131 Ancona, Italy;2. University of Maryland Center for Environmental Science, Horn Point Laboratory, P.O. Box 775, Cambridge MD 21613, USA;3. Istituto di Scienze Marine (Consiglio Nazionale delle Ricerche), Largo Fiera della Pesca, 60125 Ancona, Italy;1. Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, PO Box 123, Broadway, New South Wales 2007, Australia;2. Senckenberg Research Institute, Senckenberg am Meer, German Center for Marine Biodiversity Research (DZMB), Südstrand 44, D-26382 Wilhelmshaven, Germany;3. Institute for Marine and Antarctic Studies, University of Tasmania, Private Bag 129, Hobart, Tasmania 7001, Australia;4. Cawthron Institute, 98 Halifax Street East, Private Bag 2, Nelson 7010, New Zealand;1. Research Group on Oceanography and Plankton Ecology, Tunisian National Institute of Agronomy (INAT), 43 Avenue Charles Nicolle, IRESA- Carthage University, LR18ES41 (Tunis El Manar University), Tunis, 1082, Tunisia;2. Center for Marine Biodiversity, Exploitation and Conservation (MARBEC), Institut de Recherche pour le Développement (IRD), Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Centre National de la Recherche Scientifique (CNRS), Montpellier University, Place Eugène Bataillon, CC093, Montpellier, Cedex 5, F-34095, France;3. National Institute for Fisheries Research, Central laboratories, Bd Sidi Abderrahmane Ain Diab 2, Casablanca, Morocco;4. Faculty of Sciences of Bizerte, Laboratory of Aquatic Systems Biodiversity and Functioning, 7021 Zarzouna, Carthage University, Bizerte, Tunisia;5. Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, PO Box 2713, Doha, Qatar;6. Institut de Recherche pour le Développement (IRD), 2 Rue Des Sports-El Menzah 1, BP 434, Tunis, 1004, Tunisia;7. Tunisian National Institute of Agronomy (INAT), 43 Avenue Charles Nicolle, IRESA-Carthage University, Tunis, 1082, Tunisia;1. Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, Via S’Alberto 163, 48123 Ravenna, Italy;2. Department of Pharmacy, University of Naples “Federico II”, Via D. Montesano 49, 80131 Naples, Italy;1. IRTA, Carretera de Poble Nou, Km 5.5, 43540 Sant Carles de la Ràpita, Spain;2. Dipartimento di Chimica delle Sostanze Naturali, Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, via D. Montesano 49, 80131 Napoli, Italy |
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| Abstract: | Individuals of Ostreopsis, a genus containing potentially toxic species which affects human health, were collected during summer-autumn 2010 and 2011 from 17 sites located along the Atlantic coast of the Iberian Peninsula, a temperate area which during summer presents contrasting seawater temperatures. Ostreopsis cells were obtained by shaking macroalgae collected from rocky-shore areas bordering accessible beaches. Isolated strains and field samples were analyzed for morphological and phylogenetic characterization where sequences of the ITS1-5.8S-ITS2 region of the rDNA delineated two different species fitting Ostreopsis cf. ovata and Ostreopsis cf. siamensis. By means of calcofluor staining and scanning electron microscopy, it was observed that field samples of both species exhibited a wide and overlapping range of dorsoventral as well as width values. Those cells presented 11–18 pores/100 μm2 and were also similar concerning plates shape and size. The main differential feature between the two species was the presence of two sizes of thecal pores (0.07–0.13 μm and 0.15–0.39 μm) in Ostreopsis cf. siamensis and one size (0.24–0.56 μm) in Ostreopsis cf. ovata. A comparison of field vs. cultured cells indicated that field isolates presented larger cells than in culture. |
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| Keywords: | Iberian Peninsula ITS1-5.8S-ITS2 Morphology Phylogeny |
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