Agrobacterium rhizogenes mediated induction of apparently untransformed roots and callus in chrysanthemum |
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Authors: | Monique F. van Wordragen Pieter B. F. Ouwerkerk Hans J. M. Dons |
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Affiliation: | (1) Department of Developmental Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, The Netherlands;(2) Present address: Department of Molecular Biology, Agricultural University, Dreyenlaan 3, 6703 HA Wageningen, The Netherlands;(3) Molbas Research Group, Department of Plant Molecular Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands |
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Abstract: | The agropine type Agrobacterium rhizogenes strain LBA9402 induced callus and roots on stems of greenhouse grown plants and on leaf disks of in vitro grown plantlets of chrysanthemum (Dendranthema grandiflora Tzvel.). In this callus and roots no opines were detected, nor were any of the other features of the hairy root syndrome observed. Experiments aimed to identify the nature of the tumour-like growth revealed that induction was correlated with the presence of the TR-DNA on the Ri-plasmid. Root induction was probably the result of auxin synthesis following transient expression of iaaM and iaaH genes, present on the TR-DNA. The chrysanthemum cultivar used, cv. Parliament , showed a high auxin sensitivity compared to tobacco. Analysis of early transformation events using the GUSintron reporter gene revealed that low efficiency gene transfer and transient gene expression took place, but most probably without stable integration of the T-DNA in the plant genome. The results presented here stress the fact that callus formation or root induction as measures for transformation efficiency should be used with caution. |
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Keywords: | Agrobacterium rhizogenes auxin sensitivity Dendranthema grandiflora root induction TL-and TR-DNA-tumour proliferation |
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