人扁桃体中应激免疫抑制蛋白的初步观察

以前的实验证明,在应激条件下,外周淋巴组织中产生一种蛋白质,具有抑制某些免疫功能的作用,称为应激免疫抑制蛋白（immune suppressive protein of stress,ISPS）。本实验用人外周淋巴器官扁桃体进行了研究,证明扁桃体的提取物能抑制小鼠由Con A诱导的淋巴细胞转化,而且这种抑制作用可被ISPS单克隆抗体（2C4）部分翻转。间接ELISA法证明人扁桃体提取物能与2C4单克隆抗体相结合。以ISPS单克隆抗体（2C4）作免疫组织化学研究,证明人扁桃体中有很多染色呈阳性的细胞。这些结果从不同角度提示,人外周淋巴组织中存在一种与ISPS相类似的免疫抑制物质。

A preliminary study on the immune suppressive protein of stress in human tonsil

Our previous work demonstrated that under the conditions of restraint stress and under the control of central nervous system (CNS), an immune suppressive protein of stress (ISPS) was generated in peripheral lymph tissue and released into the blood stream, acting as an immune suppressor. In the present work, a protein similar to ISPS was found in human tonsil (a peripheral lymph tissue). Human tonsil was homogenized and the extract was prepared. It was found that lymphocyte proliferation was significantly suppressed by the extract. The suppression induced by the extract was partially reversed by the monoclonal antibody against ISPS (2C4). In ELISA test, the extract was able to bind to the monoclonal antibody. By immunohistochemistry, many ISPS positive cells were found in human tonsil. The ISPS positive cells were also found in human lymph nodes. Taken together, all the results demonstrate that a protein similar to ISPS may exist in human peripheral lymphoid tissue.