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华北落叶松体细胞胚胎发生及遗传转化实验系统的建立(简报)
引用本文:齐力旺,韩一凡,李玲,韩素英. 华北落叶松体细胞胚胎发生及遗传转化实验系统的建立(简报)[J]. 分子细胞生物学报, 2000, 33(4): 357-366
作者姓名:齐力旺  韩一凡  李玲  韩素英
作者单位:齐力旺(中国林科院林业研究所分子遗传室,北京 100091);韩一凡(中国林科院林业研究所分子遗传室,北京 100091);李玲(中国林科院林业研究所分子遗传室,北京 100091);Dietrich Ewald(Federal Research Centre fof Forestry,Insitute for Forestry Tree Breeding and Biotechnology,Eberswalder Chaussee 6,15377 Waldsieversdorf,Germany);韩素英(山西农业大学林木生物技术研究中心,太谷 030801)
基金项目:本工作为国家自然科学基金(批准号:No.39870632)和国家林业局“948”项目(批准号;No.98-4-04-02)资助。
摘    要:华北落叶松(Larix principis-Rupprechtii)是我国北方中高山地区重要的针叶速生用材树种,进行其体细胞胚胎发生和植株再生的研究,在针叶树无性快速繁殖及基因工程育种上有其特殊的用途,既可为针叶树无性系林业提供产业化途径,也可作为目的基因遗传转化实验系统。针叶树的基因转化相对较难,再生更属不易,Lelu等报道过杂种落叶松与欧洲落叶松体细胞胚胎发生方面的研究;而我国尚未见有落叶松体细胞胚胎发生的研究报道。我们

关 键 词:华北落叶松 体细胞胚胎发生 胚性细胞系 再诱导
修稿时间:1999-09-18

THE SOMATIC EMBRYOGENESIS AND ESTABLISHMENT OF TRANSFORMATION EXPERIMENT SYSTEM IN LARIX PRINCIPIS-RUPPRECHTII
Dietrich Ewald,QI Li Wang,HAN Yi Fan,LI Ling,Dietrich Ewald,HAN Su Ying. THE SOMATIC EMBRYOGENESIS AND ESTABLISHMENT OF TRANSFORMATION EXPERIMENT SYSTEM IN LARIX PRINCIPIS-RUPPRECHTII[J]. Journal of Molecular Cell Biology, 2000, 33(4): 357-366
Authors:Dietrich Ewald  QI Li Wang  HAN Yi Fan  LI Ling  Dietrich Ewald  HAN Su Ying
Affiliation:QI Li Wang,HAN Yi Fan,LI Ling;(Molecular Genetic Department,Research Institute of Forestry,Chinese Academy of Forestry Beijing 100091);Dietrich Ewald;(Federal Research Centre for Forestry,Institute for Forestry Tree Breeding and Biotechnology,Eberswalder Chaussee 6,15377 WaldsieDietrich Ewaldversdorf,Germany);HAN Su Ying;(Research Centre of Biotechnology,Shanxi Agricultural University,Taign 030801)
Abstract:Larix principis-Rupprechtii is one of the superior afforestation forest trees growing in north China. Embryogenic cultures were initiated from immature zygotic embryos of Larix principis-Rupprechtii on S culture medium containing 2, 4-D 0-2. 2mg/L, KT and BA each at 0 - 0. 8mg/L. Embryogenic calli were subcultured and multiplicated on S B culture medium containing dropping off each hormone concentration. We set up 33 steady-going embryogenic cell lines; We studied on the growth stage and genotype differences of every embryogenic cell lines ; and Finded more than 10 high-frequency somatic embryogen-esis cell lines such as 2K,2T,2I,2J,3C etc. . The number of 2T somatic embryos reaches 314/ per gram of embryogenic tissue and the number of 3Csomatic embryos is 185/per gram of embryogenic tissue. The re-induction method of Larix principis-Rupprechtii from somatic embryos was used to produce renewable embryogenic cultures and steady-going embryogenic cell lines effectively. Mature somatic embryos can germinate and develop further into plantlets when they are isolated and cultured on a hormone-free WPM culture medium. The regeneration plantlets were obtained. Furthermore, the transformation with a truncated gene of Bacillus thuringensis (B. t) were carried out, the PCR showed positive results, because of this, embryogenic cell line of Larix principis-Rupprechtii can be used for transformation experiments to support further breeding in forestry.
Keywords:Larix principis-Rupprechtii.Somatic embryogenesis.Embryogenic cell Lines.Re-induction
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