Rust mite resistance in apple assessed by quantitative trait loci analysis |
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Authors: | Sibylle Stoeckli Karsten Mody Andrea Patocchi Markus Kellerhals Silvia Dorn |
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Institution: | (1) ETH Zurich, Institute of Plant Sciences/Applied Entomology, 8092 Zurich, Switzerland;(2) ETH Zurich, Institute of Integrated Biology/Plant Pathology, 8092 Zurich, Switzerland;(3) Agroscope Changins-Wadenswil Research Station ACW, P.O. Box 185, Schloss, 8820 Wadenswil, Switzerland |
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Abstract: | The aim of this study was to assess the genetic basis of rust mite (Aculus schlechtendali) resistance in apple (Malus × domestica). A. schlechtendali infestation of apple trees has increased as a consequence of reduced side effects of modern fungicides on rust mites. An
analysis of quantitative trait loci (QTLs) was carried out using linkage map data available for F1 progeny plants of the cultivars ‘Fiesta’ × ‘Discovery’. Apple trees representing 160 different genotypes were surveyed for
rust mite infestation, each at three different sites in two consecutive years. The distribution of rust mites on the individual
apple genotypes was aggregated and significantly affected by apple genotype and site. We identified two QTLs for A. schlechtendali resistance on linkage group 7 of ‘Fiesta’. The AFLP marker E35M42-0146 (20.2 cM) and the RAPD marker AE10-400 (45.8 cM) were
closest positioned to the QTLs and explained between 11.0% and 16.6% of the phenotypic variability. Additionally, putative
QTLs on the ‘Discovery’ chromosomes 4, 5 and 8 were detected. The SSR marker Hi03a10 identified to be associated to one of
the QTLs (AFLP marker E35M42-0146) was traced back in the ‘Fiesta’ pedigree to the apple cultivar ‘Wagener’. This marker may
facilitate the breeding of resistant apple cultivars by marker assisted selection. Furthermore, the genetic background of
rust mite resistance in existing cultivars can be evaluated by testing them for the identified SSR marker.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Quantitative trait loci Apple Malus × domestica Rust mite resistance |
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