Characterization and expression of an nsLTPs-like antimicrobial protein gene from motherwort (Leonurus japonicus) |
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Authors: | Xingyong Yang Xiaowen Wang Xianbi Li Beibei Zhang Yuehua Xiao Demou Li Chengjian Xie Yan Pei |
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Institution: | (1) Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture of China and Biotechnology Research Center, Southwest University, Chongqing, 400716, China |
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Abstract: | In screening for potent antimicrobial proteins (AMPs) from plant seeds, we had purified a heat-stable AMP, LJAMP2, from the
seeds of a medicine herb, motherwort (Leonurus japonicus Houtt). In an in vitro assay, the protein can inhibit the growth of both fungi and bacteria. Then a cDNA encoding LJAMP2
was cloned by the rapid amplification of cDNA ends based on the N-terminal amino acid sequence determined. The deduced amino
acid sequences of this cDNA show similarity to plant non-specific lipid transfer proteins. Northern blotting assay revealed
that this nsLTP-like gene, designated LJAMP2, was expressed in seeds. Overexpression of LJAMP2 in tobacco enhanced resistance to the fungal pathogen Alternaria alternata and the bacterial pathogen Ralstonia
solanacearum, significantly, while no visible alteration in plant growth and development. Our data confirm the antifungal and antibacterial
function of LJAMP2 from motherwort seeds and suggest the potential of LJAMP2 in improving disease resistance in plants. |
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Keywords: | Expression Disease resistance Antimicrobial protein Phylopenetic analysis Transgenic tobacco |
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