Metabolism of tritiated and deuterated gibberellins A1, A4 and A9 in Sitka spruce (Picea sitchensis) shoots during the period of cone-bud differentiation |
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Authors: | Thomas Moritz,J. Julian Philipson,Per Christer Odé n |
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Affiliation: | Dept of Forest Genetics and Plant Physiology, Swedish Univ. of Agricultural Sciences, S-901 83 Umeå, Sweden;Forestry Commission Northern Research Station, Roslin Midlothian EH25 9SY, UK;Dept of Forest Genetics and Plant Physiology, Swedish Univ, of Agricultural Sciences, S-901 83 Umeå, Sweden |
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Abstract: | A mixture of tritiated and deuterated gibberellins (GAs) was injected into elongating shoots of Sitka spruce [ Picea sitchensis (Bong.) Carr.] grafts grown under environmental conditions that were either inductive (heat and drought, HD) or non-inductive (cool and wet, CW) for flowering. The metabolites were purified by high performance liquid chromatography (HPLC), detected by liquid scintillation counting of aliquots of collected fractions and identified by gas chromatography–mass spectrometry (GC-MS). Deuterated GA9 was converted to deuterated GA4, deuterated GA34, and deuterated GA1 in both treatments. Deuterated GA4 was metabolized to deuterated GA34 and deuterated GA1 in the CW material, but only deuterated GA1 was detected in the HD material. The amount of detected metabolites was higher in the HD material, caused by a higher rate of metabolism and/or smaller losses of the metabolites during sample purification. GA1 was converted to a polar unidentified metabolite in both treatments, but to a higher degree in the CW treatment. |
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Keywords: | Flowering gas chromatography–mass spectrometry gibberellins metabolism Picea sitchensis Sitka spruce |
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