藻蓝蛋白色素肽光动力学抗肿瘤作用的实验研究

目的：探讨藻蓝蛋白色素肽的理化特性及光动力疗法（PDT）抗肿瘤效果。方法：用柱层析法从螺旋藻藻蛋白酶介产物中分离色素肽，并用MTT法及吖啶橙染色法检测色素肽对体外培养的小鼠肉瘤细胞S180及荷瘤小鼠PD。结果：分离出三种色素肽的分子量分别为17.4KD,7.72KD及6.6KD，它们的吸收光谱峰值分别位于618nm，580nm,605nm，荧光发射峰均在685nm处。MTT法检测证明，在浓度100μg/ml，用波长为580nm,600nm，照射剂量为28.8J/cm^2染料激光（氩离子为激光光源）照射，对肿瘤细胞的杀伤率达69．2％－80．2％;在色素肽CCP1，CCP3介导的PDT对小鼠移植瘤生长影响的实验发现，色素肽易被瘤细胞吸收，当激光照射剂量为120J/cm^2，肿瘤直径在0.5-0.7cm范围内，瘤体旁注射剂量为50μg的CPP1及CCP3光敏剂时，藻胆蛋白酶介产的的抑瘤率可达46％－81％，且被PDT作用后的细胞表现了典型的凋亡细胞特征。

Antitumor Studies of C-Phycocyanin Chromophore Peptides Mediated Photodynamic Therapy

Objective: to study the antitumor effects and mechanisms of C-Phycocyanin chromophore peptides mediated Photodynamic therapy in vitro and in vivo.Methods: Chromophore peptides CCP 1～CCP 3 were isolated by DEAE-Sepharose Fast Flow, chracterized with respect to spectroscope properties and molecular weight. The PDT effects of CCP 1～CCP 3 on S180 cell and tumor bearing mice were assessed with MTT method and acridine orange staining. Result: CCP 1 has a molecular weight of 17400, CCP 2 7720, CCP 3 6600, their absorption spectra with maximum are located at 618 nm, 580 nm and 600 nm separately. In vitro test, the highest inhibited rates of of S180 cells were 19.8 % ～30.8 %, when concentration of chromophore peptides was 100μg / mL, irradiated by dying laser with dose of 28.8 J/cm 2. In vivo test, in the condition of tumor diameter between 0.5 cm～ 0.7 cm and the irradiation dose of 120 J/cm 2, the tumor inhibited rates were 46 %～81 % , the tumor cells inhibited by PDT showed typical apoptosis character.