| Abstract: | The motility of sperm was examined in vivo in the vas deferens, the spermatophore, and the spermatheca of the red-spotted newt and in in vitro preparations with variations in osmolality, hydrogen ion concentration, and concentrations of specific osmolytes. Sperm were motile within the spermatophore, but little or no evidence of motility was seen in the spermatheca or the vas deferens. Approximately 25% of sperm from the vas deferens became motile when dispersed in spermatic fluid plasma, the sperm-bearing liquid of the vas deferens, indicating crowding to be a possible motion-restraining factor. Fewer than 50% were motile in several saline media isosmotic with spermatic fluid plasma, whereas more than 90% became motile in distilled water or media at osmolalities near that of pond water. Motility in isosmotic solutions persisted beyond 12 hours, but at low osmolality ceased by 6 hours. When dispersed at higher osmolalities initial motility was low but increased to isosmotic levels by 12 hours. Responses to immersion in solutions of mannitol were similar to ones observed in saline solutions of equivalent osmolality. Dispersion in hydrogen ion concentrations between pH 4 and 9 did not affect the initial motility of sperm, but after 12 hours at pH 9, pH 4 or 5 movement was inhibited. In general, these data indicate a major role for osmolality in the enforced quiescence of sperm during storage and demonstrate that the low osmolality of pond water is primarily responsible for the activation of sperm in the spermatophore of the newt. |
