Characterization of extracellular polymeric substances from denitrifying organism Comamonas denitrificans |
| |
Authors: | Sofia Andersson Gunnel Dalhammar Carl Johan Land and Gunaratna Kuttuva Rajarao |
| |
Institution: | (1) Division of Environmental Microbiology, School of Biotechnology, Royal Institute of Technology, AlbaNova University Centre, 106 91 Stockholm, Sweden;(2) KTH-BIO, AlbaNova University Centre, 106 91 Stockholm, Sweden |
| |
Abstract: | Extracellular polymeric substances (EPS) play an important role in the formation and activity of biofilms in wastewater treatment
(WWT). The EPS of the denitrifying biomarker Comamonas denitrificans strain 110, produced in different culture media and growth modes, were characterized. The EPS mainly contained protein (3–37%),
nucleic acids (9–50%), and carbohydrates (3–21%). The extracellular DNA was found to be important for initial biofilm formation
since biofilm, but not planktonic growth, was inhibited in the presence of DNase. The polysaccharide fraction appeared to
consist of at least two distinct polymers, one branched fraction (A) made up of glucose and mannose with a molecular weight
around 100 kDa. The other fraction (B) was larger and consisted of ribose, mannose, glucose, rhamnose, arabinose, galactose,
and N-acetylglucosamine. Fraction B polysaccharides were mainly found in capsular EPS which was the dominant type in biofilms and
agar-grown colonies. Fraction A was abundant in the released EPS, the dominant type in planktonic cultures. Biofilm and agar-grown
EPS displayed similar overall properties while planktonic EPS showed clear compositional disparity. This study presents results
on the physiology of a key WWT organism, which may be useful in the future development of improved biofilm techniques for
WWT purposes. |
| |
Keywords: | Biofilm Comamonas denitrificans GC– MS Extracellular polymeric substances HPAEC Polysaccharides |
本文献已被 SpringerLink 等数据库收录! |
|