Deletion mutants of the B type natriuretic peptide receptor: cDNA expression and protein purification and characterization |
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Authors: | O V Solov’eva D L Kakuev N V Gibanova V M Lipkin |
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Institution: | (1) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117871 GSP-7 Moscow, Russia |
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Abstract: | The C type natriuretic peptide (CNP) is a peptide hormone stimulating vasorelaxation and inhibiting cell proliferation. CNP
activates the type B natriuretic peptide receptor (NPR-B), known as the guanylate cyclase B membrane enzyme, which results
in the cGMP release. To study functional properties of NPR-B, its gene fragments were expressed in methylotrophic yeastsPichia pastoris. Conditions were found providing for secretion of functionally active recombinant proteins NPR-Bs and NPR-Bl into the cultural
medium in a yield of 25 mg/l culture. Their specific activity was 0.97 and 0.93 μmol cGMP min−1 mg−1 protein, respectively. It was shown that NPR-B belongs to the family of Ser/Thr protein kinases and can be autophosphorylated
at the serine residues. |
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Keywords: | bovine retina heterologous expression natriuretic peptide receptor yeasts |
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