Decreased total carbonic anhydrase esterase activity and decreased levels of carbonic anhydrase 1 isozyme in erythrocytes of type II diabetic patients |
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| Authors: | Kanwal K. Gambhir Jehan Ornasir Verle Headings Adolphus Bonar |
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| Affiliation: | (1) Molecular Endocrinology Laboratory, Department of Medicine, College of Medicine, and Department of Genetics and Human Genetics, Graduate School, Howard University, Washington, DC 20060, USA;(2) Department of Genetics and Human Genetics, Graduate School, Howard University, Washington, DC 20059, USA;(3) Department of Genetics and Human Genetics, College of Medicine and Graduate School, Howard University, Washington, DC 20059, USA;(4) Divison of Endocrinology and Metabolism, Department of Medicine, College of Medicine, and Department of Genetics and Human Genetics, Graduate School, Howard University, Washington, DC 20060, USA |
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| Abstract: | In this exploratory study, we investigated total erythrocyte carbonic anhydrase (CA) estrase activity as well as CA I isozyme concentration in patients with diabetes mellitus type II (DM) and healthy individuals of Howard University Hospital community. Total estrase activity of CA was measured spectrophotometrically using p-nitrophenol acetate before and after inhibition with acetazolamide. CA I isozyme was measured by radial immunodiffusion using monoclonal antibody (CA I) in agarose plates. The study involved 20 consented participants; 10 normal (N) and 10 (DM), 21 to 84 years of age. The study was approved by the Howard University Institution Review Board. The CA activity was measured following lysis of cells as U/min/mL and CA I concentration as mg/l. We observed CA activity as 46.3±4(N) and 25±2.1 (DM) whereas CA I concentration as 1896±125 (N) and 1104 ±63 (DM). We speculate that the change in the CA activity may of fundamental importance in the regulation of intracellular; pHi for the basic control of metabolism in diabetes mellitus. Further, we propose that CA activity is a good candidate for a biomarker of diabetes mellitus for the early detection of insulin resistance because the CA activity variation was proportional to the severity of the diabetes. Jehan Ornasir—these studies were undertaken as a partial requirement of her M.S. Degree, Graduate School, Howard University, Washington, DC, USA |
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| Keywords: | Carbonic anhydrase carbonic anhydrase I isozyme diabetes mellitus erythrocyte human red blood cell |
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