Introduction of lanthanide(III) chelates to oligopeptides on solid phase |
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| Authors: | Peuralahti Jari Hakala Harri Mukkala Veli-Matti Loman Kristiina Hurskainen Pertti Mulari Outi Hovinen Jari |
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| Affiliation: | PerkinElmer Life Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland. |
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| Abstract: | The synthesis of oligopeptide building blocks for the introduction of nonluminescent and luminescent lanthanide(III) chelates to the oligopeptide structure on the solid phase is described. The oligopeptide conjugates synthesized were used in DELFIA-based receptor binding assay (motilin) as well as in LANCE time-resolved fluorescence quenching assay (caspase-3). |
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