A refined method for silver staining of nucleic acids fixed on nitrocellulose |
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| Authors: | X Su |
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| Affiliation: | Department of Biological Sciences, University of California, Santa Barbara 93106. |
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| Abstract: | A refined silver staining method was developed to stain nucleic acids fixed onto nitrocellulose membranes and nylon-based membranes. Approximately 4 ng RNA or DNA can be stained with this method with no protein interference. This method involves simple repetition of immersions of membranes in three solutions prepared from common chemicals. The total staining time is less than 30 min. |
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