Identification of two glutaminases inRhizobium etli |
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Authors: | Socorro Durán Luis Sánchez-Linares Alejandro Huerta-Saquero Gisela Du Pont Alejandra Huerta-Zepeda Jorge Calderón |
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Institution: | 1. Departamento de Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510, México D.F., México
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Abstract: | We present evidence thatRhizobium etli has two glutaminases differentiated by their thermostability and electrophoretic mobility. The thermostable glutaminase (B) is constitutive, in contrast with the thermolabile glutaminase (A), which is positively regulated by glutamine and negatively regulated by ammonium and by the carbon source. In distinction to glutaminase A, glutaminase B plays a minor role in the utilization of glutamine as a carbon source, but it may play a role in maintaining the balance of glutamine and glutamate. By complementation of theRhizobium etli LM16 mutant that lacks glutaminase A, we have cloned the gene that codes for this enzyme. |
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