Localisation of CP43 in single molecules of photosystem 2 using protein deletion and scanning tunnelling microscopy |
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| Authors: | C S Barton P B Lukins |
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| Institution: | (1) Present address: School of Physics A28, University of Sydney, NSW, 2006, Australia;(2) National Measurement Institute, P.O. Box 264, Lindfield, NSW, 2070, Australia |
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| Abstract: | Scanning tunnelling microscopy of intact D1/D2/CP47/CP43 photosystem 2 (PS2) core complexes and CP43-deleted D1/D2/CP47 core
complexes shows definitively that the CP43 subunits reside at the ends of the dimeric core complex. The CP43-removal procedure
produces CP43-deleted cores with minimal conformational distortion to the D1/D2/CP47 residual core complex. There was excellent
agreement between the X-ray and STM structures for the intact core complex, and between the STM image for the CP43-deleted
core complex and the X-ray model with the components assigned to CP43 omitted. |
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| Keywords: | CP43 deletion model STM X-ray structure |
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