Genetic inactivation of the psaB gene in Synechocystis sp. PCC 6803 disrupts assembly of photosystem I |
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Authors: | Lawrence B. Smart Lee McIntosh |
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Affiliation: | (1) M.S.U.-D.O.E. Plant Research Laboratory, Michigan State University, 48824-1312 East Lansing, MI, USA;(2) Genetics Program, Michigan State University, 48824-1312 East Lansing, MI, USA;(3) Department of Biochemistry, Michigan State University, 48824-1312 East Lansing, MI, USA |
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Abstract: | The reaction center of photosystem (PS) I is comprised of a heterodimer of homologous polypeptides, PsaA and PsaB. In order to investigate the biogenesis of PS I, the psaB gene was inactivated by targeted mutagenesis in the unicellular cyanobacterium Synechocystis 6803. This mutation resulted in disruption of stable PS I assembly, but PS II assembled normally. Expression of the psaA gene was not affected by the mutation, but PsaA protein was not detected, indicating that stable PsaA homodimers did not form. The ability to inactivate psaB makes it a viable target for site-directed mutagenesis. |
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Keywords: | photosystem I psaB Synechocystis 6803 targeted mutagenesis |
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