Differentiated properties characteristic of renal proximal epithelium in a cell line derived from a normal monkey kidney (JTC-12) |
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Authors: | Yoh Takuwa Etsuro Ogata |
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Institution: | (1) Fourth Department of Internal Medicine, University of Tokyo School of Medicine, 112 Tokyo, Japan;(2) Division of Endocrinology, Department of Internal Medicine, Yale University School of Medicine, 333 Cedar Street, 06510 New Haven, CT |
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Abstract: | Summary The JTC-12 cell, an established cell line derived from a normal monkey kidney, was studied in an attempt to characterize the
epithelial qualities. Phase contrast microscopy showed dome formation in confluent monolayers and electron microscopic examinations
revealed the presence of numerous microvilli on the apical membranes and desmosome between cells. Sonicated cells showed activities
of γ-glutamyl transpeptidase, leucine aminopeptidase, alkaline phosphatase, and trehalase, marker enzymes of renal proximal
epithelium. Alkaline phosphatase activity exhibited the characteristics of a renal type isozyme. Furthermore, confluent JTC-12
monolayers exhibited Na+-dependent transport of hexose, amino acid as well as inorganic phosphate. These findings indicate that JTC-12 cells in monolayer
culture maintain ultrastructural, biochemical, and physiological properties of renal proximal epithelial cells. This cell
line will be useful for further studies on cellular functions of renal proximal epithelium.
This work was supported in part by grants from The Ministry of Health and Welfare of Japan and from the Ministry of Education,
Science and Culture of Japan. |
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Keywords: | monkey kidney cells renal proximal epithelium dome formation microvilli membrane transport enzyme activities |
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