Effect of surgically induced cholestasis on the levels of hepatic zinc and metallothionein in rat liver |
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| Authors: | E Brambila J L Munoz-Sanchez M P Waalkes A Albores |
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| Institution: | (1) Facultad de Ciencias Quimicas, Benemerita Universidad, Autonoma de Puebla, Mexico;(2) Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico;(3) Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, National Cancer Institute at National Institute of Environmental Health Sciences, 27706 Research Triangle Park, NC;(4) Departamento de Farmacologia y Toxicologia, Seccion de Toxicologia Ambiental, Centro de Investigacion y Estudios, Avanzados del IPN, Mexico;(5) Present address: Inorganic Carcinigenesis Section, NCI at NIEHS, 111 Alexander Drive, Bldg. 101 SC MD:FO-09, RM F095, 27706 Research Triangle Park, NC |
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| Abstract: | Early effects of experimental cholestasis on the homeostasis of zinc (Zn) and metallothionein (MT) were studied in rats which
had undergone bile duct ligation for 0, 3, 6, 9, 12, 16, 20, and 24 h. Transient increases in hepatic Zn levels were observed
at 9 h but returned to control values at 12 h. Serum Zn levels increased at 24 h. Cholestasis was confirmed by increased serum
alkaline phosphatase (AP) activity. MT increased at 3 h and reached a maximum level at 12 h and remained elevated even at
24 h after the onset of experimental cholestasis. No hepatocellular damage was detected according to the results of alanine
aminotransferase (ALT) activities in serum. These results shown that the increases in Zn observed in liver are related to
bile stagnation rather than to a hepatocellular damage and that increased MT occurs concurrently with increased hepatic Zn.
These observations suggest that the cellular levels of Zn in cholestasis is regulated by homeostatic mechanisms, of which
one could be mediated by MT. |
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| Keywords: | Zinc metallothionein cholestasis |
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