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Cyclic (1 → 2)-β-d-glucans excreted by the glucuronan-producing strain Rhizobium meffloti M5N1CS CS (NCIM B 40472) and by the succinoglycan-producing strain Rhizobium meliloti M5N1
Authors:P Michaud  J Courtois  B Courtois  A Heyraud  P Colin-Morel  J-P Sguin  J-N Barbotin
Institution:

Laboratoire de Biotechnologie Microbienne, Université de Picardie Jules Verne, Avenue des Facultés, Le Bailly, 80025 Amiens Cedex 1, France

Centre de Recherches sur les Macromolécules Végétales (CNRS), SP 53X, 38041 Grenoble Cedex, France

Laboratoire de Génie Cellulaire, Faculté des Sciences, Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex 1, France

Abstract:During fermentation, the mutant strain Rhizobium mefliloti M5N1 CS, which induces nodule formation on alfalfa roots, produces a partially acetylated (1 → 4)-β-d-glucuronan. In addition to this exopolysaccharide of high molecular weight, the mutant strain produces oligoglucoronates and cyclic (1 → 2)-β-d-glucans with degrees of polymerization from 17 to 30. Under the conditions applied, magnesium has no effect on cyclic glucan production by the mutant strain, but the succinoglycan production by the wild-type strain Rhizobium meliloti M5N1 increases.
Keywords:polysaccharide  Rhizobium meliloti  cyclic (1 → 2)-β-d-glucans
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