Assessment system for dioxin absorption in the small intestine and prevention of its absorption by food factors |
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Authors: | Natsume Yayoi Satsu Hideo Kitamura Kazushige Okamoto Naoto Shimizu Makoto |
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Affiliation: | Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. |
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Abstract: | It has been reported that 90% of the amount of dioxin in the whole body is absorbed orally with food. However, a concise and simple system to assess dioxin absorption in the small intestine has not yet been established. The present study reports a new in vitro assessment system for this purpose. A stable dioxin-responsive cell line was established by introducing a plasmid that incorporates a xenobiotic-responsive element upstream of the luciferase gene into human hepatic HepG2 genomic DNA. Dioxin was added to the apical side of differentiated human intestinal epithelial Caco-2 cell monolayers that had been cultured on a semipermeable membrane, and the basal medium was recovered after an appropriate incubation time. To the recovered medium was added dioxin-responsive HepG2, and a luciferase assay was performed. The established stable cell line clearly showed dose-and time-dependent response to dioxin. When a food factor such as chlorophyll, which has been reported to increase dioxin excretion in in vivo studies, was added with dioxin, a significant decrease in dioxin permeability to the Caco-2 monolayer was observed. This assessment system would be useful to search for those food factors that could prevent dioxin absorption in the small intestine. |
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