向日葵幼胚培养中体细胞胚胎发生的观察

1.向日葵不同品种体细胞胚胎发生的情况不同。2.较高浓度的蔗糖有利于向日葵幼胚的体细胞胚胎发生。3.在同样条件下,2mm长的幼胚较其它时期的幼胚体细胞胚胎发生的频率高。4.在蔗糖浓度为17.5％并分别加入0.5—10.0ppm玉米素的Nitsch培养基中,向日葵幼胚产生体细胞胚胎发生的频率随着玉米素浓度的增高而增加。5. 2,4-D能使体细胞胚胎发生,但不能分化器官。6.切片观察表明:在含玉米素的培养基上,幼胚产生了胚性细胞团和胚状体。并多数发生于子叶与下胚轴的深层。胚性细胞团周围细胞退化,使其与周围组织之间形成间隙。

OBSERVATION OF SOMATIC EMBRYOGENESIS IN THE IMMATURE EMBRYO CULTURE OF SUNFLOWER

1. The somatic crnbryogencsis is produced differently in the different cultivars of Sunflower. 2. The higher concentration of sucrose makes better somatic cmbryogcnesis of Sunflower immature embryos. 3. The immature embryos of 2 mm length among the embryos of different size have the highest percentage somatic embryogenesis in the same condition. 4. The higher concentration of Zeatin is, the more percentage of somatic embryogenesis in Nitsch medium supplemented with 0.5-10.0ppm Zeatin and 17.5% sucrose in the immature embryo culture of Sunflower. 5. 2,4 -D makes the embryos somatic embryogenesis, but does not make them differentiation. 6. In the slides show, the immature embryos producte the embryonic cell groups and embryoids, many of which are emerged in the cotyledon and hypocotyledonary axis. The surrounding cells of the embryonic cell groups degenerate, such there is the space between the groups of cell and the surrounding tissue.