Characterization of a newin vitro model for studies of reepithelialization in human partial thickness wounds |
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Authors: | Katarina Jansson Gunnar Kratz Anders Haegerstrand |
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Institution: | (1) Department of Neuroscience, Karolinska Institutet, S-171 77 Stockholm, Sweden |
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Abstract: | Summary Reepithelialization of artificial partial thickness wounds made in biopsies of human skin was determined after 3, 5, or 7
d of incubation, submerged or elevated to the air-liquid interface. The biopsies were reepithelialized within 5–7 d, with
a more complete epidermal healing in wounds exposed to air. Both types of wounds showed similar time-course in deposition
of basement membrane components, as detected by immunofluorescence labeling. Laminin and collagen type VII were deposited
underneath the migrating tips, whereas collagen type IV was detected after reepithelialization. Markers of terminal differentiation
showed a pattern close to normal in the air-liquid incubated wounds after reepithelialization. Involucrin was detected in
the suprabasal regions of the migrating epidermis and thereafter in the upper half of neo-epidermis in the air-liquid incubated
wound. Filaggrin could not be detected in the submerged wounds at any time during healing, whereas wounds exposed to air showed
a well-differentiated epidermis by Day 7. Tritiated thymidine-incorporation indicated proliferation of epidermal and dermal
cells during reepithelialization and a maintained viability, as shown by cultivation of endothelial- and fibroblast-like cells
obtained from the dermis 7 d after wounding.
Reepithelialization in this humanin vitro model is supported by a matrix close to normal with the possibility of extracellular influences and cell-cell interactions
and, in addition, the technique is simple and reproducible. Therefore, we suggest this model for studies of regeneration in
culture and as a complement toin vivo studies on epidermal healing. |
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Keywords: | reepithelialization basement membrane epidermal-dermal junction terminal differentiation |
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