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E.coli分泌表达载体的构建和人表皮生长因子在E.coli中的分泌性表达
引用本文:张宏权,王允玲,周廷冲,王会信,刘农乐,蒋滋慧.E.coli分泌表达载体的构建和人表皮生长因子在E.coli中的分泌性表达[J].中国生物化学与分子生物学报,1995,11(4):371-376.
作者姓名:张宏权  王允玲  周廷冲  王会信  刘农乐  蒋滋慧
作者单位:军事医学科学院基础医学研究所
摘    要:采用PCR技术从E.coli基因组片段中克隆出碱性磷酸酯酶(PhoA)的启动子和信号肽序列.在PhoA启动予5'端设计了EcoRⅠ酶切位点,在信号肽编码序列3'端设计了HindⅢ酶切位点.将PCR产物酶切后EcoRⅠ-HindⅢ片段克隆至pBR322的EcoRⅠ-HindⅢ位点,组构出含有PhoA启动子和信号肽序列的分泌表达载体pBM-Pho-1.之后将人表皮生长因子的成熟肽基因克隆至该载体,使之在E.coli中获得分泌表达,另采用pINⅢ载体系统以分泌方式表达了人表皮生长因子。

关 键 词:人表皮生长因子  E.coli分泌表达载体  基因表达  
收稿时间:1995-08-20

Construction of E. coli Secretion Vector and the Secretory Expression of Human Epidermal Growth Factor in E. coli
Zhang,Hong-Quan,Wang,Yun-Ling,Zhou,Ting-Chong,Wang,Hui-Xin,Liu Nong-Le,Jiang,Zi-Hui.Construction of E. coli Secretion Vector and the Secretory Expression of Human Epidermal Growth Factor in E. coli[J].Chinese Journal of Biochemistry and Molecular Biology,1995,11(4):371-376.
Authors:Zhang  Hong-Quan  Wang  Yun-Ling  Zhou  Ting-Chong  Wang  Hui-Xin  Liu Nong-Le  Jiang  Zi-Hui
Institution:(Beijing Instdute of Basic Medical Sciences,Beijing 100850
Abstract:The E. coli secrection vector, PBMPho-1 ,had been constructed by amplified the E. coli alkaline phosphatase A (PhoA) promoter-signal sequences by PCR and by subcloning the PCR product, which has EcoRⅠcleavage site at its 5'terminus and has Hind Ⅲ cleavage site at its 3'terminus, into PBR322 precutted by EcoRⅠand Hind Ⅲ. Human epidermal growth factor(hEGF) mature peptide coding sequence was then cloned into the secrtion vector and was expressed in E. coli. The hEGF was also expressed in E. coli by using PIN Ⅲ secretion expression system.
Keywords:Human epidermal growth factor  E  coli secretion vector  Gene expression  
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