Differential expression of long noncoding RNAs in congenital microtia |
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| Institution: | 1. Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan;2. Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan;3. Department of Biotechnology, Asia University, Taichung, Taiwan;4. School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan;5. Institute of Clinical and Community Health Nursing, National Yang-Ming University, Taipei, Taiwan;6. Department of Obstetrics and Gynecology, School of Medicine, National Yang-Ming University, Taipei, Taiwan;7. Taiji Fetal Medicine Center, Taipei, Taiwan;8. Gene Biodesign Co. Ltd., Taipei, Taiwan;9. Department of Bioengineering, Tatung University, Taipei, Taiwan;1. Department of Burns and Plastic Surgery, Second Hospital of Shandong University, Jinan, Shandong Province, People''s Republic of China;2. Department of Auricular Reconstruction, Plastic Surgery Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing, People''s Republic of China;1. Medical Genetics Unit, Hospital Pediátrico, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal;2. Manchester Centre for Genomic Medicine, Institute of Human Development, Faculty of Medical and Human Sciences, University of Manchester, Manchester, UK;3. Mersey Regional Genetic Service, Alder Hey Hospital, Liverpool, UK;4. Central Manchester University Hospitals NHS Foundation Trust as part of Manchester Academic Health Science Centre (MAHSC), Manchester, UK;5. Unidade Hematologia Molecular, Serviço de Hematologia, CHUC, Portugal;6. Faculdade de Medicina da Universidade de Coimbra, Laboratório de Citogenética e Genómica – Faculdade de Medicina, Universidade de Coimbra, Coimbra, Portugal;7. Genomic Diagnostics Laboratory, Manchester Centre for Genomic Medicine, Central Manchester University Hospitals NHS Foundation Trust, Saint Mary''s Hospital, USA;1. Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, Guangdong, PR China;2. Department of Biochemistry, Zhongshan Medical School, Sun Yat-Sen University, Guangzhou, Guangdong, PR China;3. Key Laboratory of Reproductive Medicine of Guangdong Province, Guangzhou, Guangdong, PR China;4. Department of Blood Transfusion, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, PR China;5. Department of Cell Biology, Zhongshan Medical School, Sun Yat-Sen University, Guangzhou, Guangdong, PR China;6. Center for Reproductive Medicine, Key Laboratory for Reproductive Medicine of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, PR China;7. Department of Histology and Embryology, School of Medicine, Jinan University, Guangzhou, PR China;8. Center for Neurobiology, Zhongshan School of Medicine, Sun-Yat Sen University, Guangzhou, Guangdong, PR China;9. Department of Anatomy and Neurobiology, Zhongshan Medical School, Sun Yat-Sen University, Guangzhou, Guangdong, PR China;10. Department of Gastrointestinal-Pancreatic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, PR China |
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| Abstract: | ObjectiveTo analyse lncRNA expression profiles in microtia using bioinformatics analysis.MethodsWe examined lncRNA expression profiles in residual ear cartilage and normal ear cartilage from individual congenital microtia patients.ResultsThe gene chips used in this study included 30586 lncRNAs and 26109 mRNA probes. Intotal, 180 lncRNAs with differential expression weredetected in the residual ear cartilage compared with the normal cartilage, including 74 up-regulated and 106down-regulated lncRNAs. Signalling pathway analysis highlighted glyceride metabolism, osteoclast differentiation, andtumour growth. The results of qRT-PCR analysis were consistent with those of themicroarray.ConclusionDifferential expression of lncRNAs occurs in microtia. These lncRNAs and related signalling pathways may play an important role in the occurrence and development ofmicrotia. |
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| Keywords: | Microtia lncRNAs Microarray |
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