| 利用基因芯片对一高保守基因进行肿瘤相关分析 |
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| 引用本文： | 程超,周宗祥,徐明,赵炜,徐坚,曾立,黄燕,吴奇涵,戴建锋,应康,谢毅,毛裕民. 利用基因芯片对一高保守基因进行肿瘤相关分析[J]. 遗传, 2002, 24(3): 227-231 |
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| 作者姓名： | 程超 周宗祥 徐明 赵炜 徐坚 曾立 黄燕 吴奇涵 戴建锋 应康 谢毅 毛裕民 |
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| 作者单位： | 复旦大学,生命科学院,遗传学研究所,上海,200433 |
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| 基金项目： | 国家自然科学基金 ( 30 0 70 80 6 ) |
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| 摘 要： | 本对大规模人类cDNA测序过程中获得的一条高保守基因进行了初步功能研究，生物信息学研究发现该基因在人类、小鼠、果蝇、拟南芥和裂殖酶母中都有很高的保守性，其他分析预测该基因可能具有肿瘤相关性。RT－PCR分析表明，该基因在成人和胎儿组织中广泛谱表达。利用基因芯片分析该基因在7例肝癌、5例胰腺癌、2例喉癌和2例肺癌中表达情况，结果证实了该基因的肿瘤相关性，并且提示该基因在不同类型中可能处于不同的地位。
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| 关 键 词： | 基因芯片 高保守基因 肿瘤相关分析 反转录－PCR 结构域 |
| 文章编号： | 0253-9772(2002)03-0227-05 |
| 修稿时间： | 2001-08-27 |
Tumor Relevance Analysis of A Highly Conserved Gene by Using Gene Microarray Hybridization |
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| CHENG Chao,ZHOU Zong xiang,XU Ming,ZHAO Wei,XU Jian,ZENG Li,HUANG Yan,WU Qi han,DAI Jian feng,YING Kang,XIE Yi,MAO Yu ming. Tumor Relevance Analysis of A Highly Conserved Gene by Using Gene Microarray Hybridization[J]. Hereditas, 2002, 24(3): 227-231 |
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| Authors: | CHENG Chao ZHOU Zong xiang XU Ming ZHAO Wei XU Jian ZENG Li HUANG Yan WU Qi han DAI Jian feng YING Kang XIE Yi MAO Yu ming |
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| Affiliation: | Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China. chengcao12@263.net |
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| Abstract: | Preliminary function research of a highly conserved human gene,which was cloned from human fetal cDNA library during large-scale cDNA sequencing,is illustrated in this article.Bioinformatics analysis indicates that this gene is highly conserved in human?mouse?fruit fly?thaliana and fission yeast.Other bioinformatics analysis implies its relevance with tumors.RT-PCR analysis shows its wide-ranging expression patterns.Its expression in 16 cancer cases(including 7 liver cancer cases?5 pancreas cancer cases?2 larynx cancer cases and 2 lung cancer cases) is studied by using gene microarray analysis.The result shows its relevance with tumors and implies it may have different status in different classification of tumors. |
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| Keywords: | gene microarray conservation RT-PCR domain |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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