Sequences of initiator and elongator methionine tRNAs in bean mitochondria |
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Authors: | Laurence Marechal Pierre Guillemaut Jean-Michel Grienenberger Geneviève Jeannin Jacques-Henry Weil |
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Institution: | (1) Institut de Biologie Moléculaire et Cellulaire, Université Louis Pasteur, 15 Rue Descartes, 67084 Strasbourg Cedex, France |
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Abstract: | Summary Two bean mitochondria methionine transfer RNAs, purified by RPC-5 chromatography and two-dimensional gel electrophoresis, have been sequenced usingin vitro post-labeling techniques.One of these tRNAsMet has been identified by formylation using anE. coli enzyme as the mitochondrial tRNAF
Met. It displays strong structural homologies with prokaryotic and chloroplast tRNAF
Met sequences (70.1–83.1%) and with putative initiator tRNAm
Met genes described for wheat, maize andOenothera mitochondrial genomes (88.3–89.6%).The other tRNAMet, which is the mitochondrial elongator tRNAF
Met, shows a high degree of sequence homology (93.3–96%& with chloroplast tRNAm
Met, but a weak homology (40.7%) with a sequenced maize mitochondrial putative elongator tRNAm
Met gene.Bean mitochondrial tRNAF
Met and tRNAm
Met were hybridized to Southern blots of the mitochondrial genomes of wheat and maize, whose maps have been recently published (15, 22), in order to locate the position of their genes. |
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Keywords: | mitochondrial tRNAs plant mitochondria tRNAF
Met tRNAm
Met |
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